F march 2014 truseq rna sample preparation v2 guide. This protocol explains how to prepare up to 96 libraries starting from genomic. Documentation means illuminas user manual for this product, including. The truseq dna pcrfree library prep workflow protocol includes the. Truseq dna sample preparation low throughput lt protocol. Truseq dna pcrfree simple prep for sequencing complex. Truseq dna pcr free reference guide 000039279 instructions for preparing libraries using the truseq dna pcr free workflow. Table truseq dna pcrfree lt sample prep kit indexed adapter sequences. This guide documents the truseq dna pcrfree library prep protocol using a. This document describes how to set up the automated truseq pcr free protocol for the agilent ngs workstation option b created at ngi stockholm.
Simple, streamlined wholegenome sequencing library preparation that provides accurate and comprehensive coverage of complex genomes. This version of the pcr free protocol is not in use anymore at ngi but has since long been replaced by a version that utilise our custom extended bravo configuration. The workflows are tunable to a variety of read lengths and are supported on all illumina sequencing instruments. Product documentationavailable for download in pdf from the illumina website. Researchers can sequence a wide variety of organisms, from small genomes such as bacteria to human whole genomes. Pcrfree protocol accelerates the truseq dna library preparation workflow. Truseq dna sample preparation guide the mgh nextgen. This guide documents the sample preparation protocol using the illumina. Rnasednasefree multichannel reagent reservoirs, disposable. Documentation means illuminas user manual for this product, including without.
Web to pdf convert any web pages to highquality pdf. Product documentation in pdf is available for download from the illumina website. Within our wholegenome sequencing workflows, truseq dna pcr free offers superior coverage of areas that are traditionally difficult to sequence, such as gcrich regions, promoters, and repetitive content. Documentation means illuminas user manual for this product, including without limitation. Use pdf download to do whatever you like with pdf files on the web and regain control. Truseq rna sample preparation guide the mgh nextgen. Truseq dna pcr free provides simple, allinclusive library preparation for wholegenome sequencing applications. Dilute the total rna with nucleasefree ultra pure water to a final volume of 50. Truseq rna sample preparation low throughput lt protocol.
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